Antioxidant Activity of Tahongai Leaves (Klenhovia hospital L.) Infusa Using DPPH Method

  • Nurillahi Febria Leswana STIKES Dirgahayu Samarinda, Samarinda, Indonesia
  • Sister Sianturi STIKES Dirgahayu Samarinda, Samarinda, Indonesia
Keywords: Infusa, Kleinhovia hospita Linn, Antioxidant, Tahongai leaves, DPPH

Abstract

Tahongai plant (Kleinhovia hospita Linn.) is a plant that grows naturally on the edge of Indonesian rivers, especially in East Kalimantan. Tahongai plants are used as medicine by the Dayak people in Kalimantan, namely that empirically the use of tahongai plants is effective as a medicine that can treat various diseases such as jaundice, hypertension, diabetes, and cholesterol by drinking the boiled water. The active substances contained in tahongai leaves are saponins, flavonoids, and alkaloids are expected to have antioxidant activity. The purpose of this study was to measure the antioxidant activity of tahongai leaf infusa. The extraction method used in this study was infusa while the radical specimen used was DPPH with Vitamin C as the comparison. Phytochemical screening was also carried out ontahongai leaf infusa extract positively containing flavonoids, tannins, saponins and phenols. Based on the results of the DPPH radical scavenging analysis of Tahongai leaf infusion, the IC50 value was 139.51 mg/L with the medium antioxidant activity.

References

[1] Saputra, H. S., dan Sampepana, E., Pembuatan Sirup Dari Ekstrak Tahongai (Klienhovia hospital Linn.), Proseding Seminar Nasional Hasil Riset dan Standardisasi Industri VI, Hal. 63-70. Balai Riset dan Standardisasi Industri Banda Aceh, 2016.
[2] Khairul. Amry, Formulasi dan Uji Stabilitas Fisik Sediaan Sirup Kombinasi Ekstrak Air Daun Tahongai (Kleinhovia hospita L.) dan Rimpang Temulawak (Curcuma xanthorriza Roxb.), Universitas Hasanuddin, Makassar, pp. 6–7, 2014.
[3] Tristantini, D., Ismawati, A., Pradana, B. T., Jonathan, J. G., “Pengujian Aktivitas Antioksidan Menggunakan Metode DPPH pada Daun Tanjung (Mimusops elengi L)”, Prosiding Seminar Nasional Teknik Kimia, 2016.
[4] Sie, J. O., “Daya Antioksidan Ekstrak Etanol Kulit Manggis (Gracinia mangostana Linn) Hasil Pengadukan dan Refluk”, Jurnal Ilmiah Mahasiswa, 2013; vol. 2, no. 1.
[5] Widiastuti, N., “Pengukuran Aktivitas Antioksidan dengan Metode CUPRAC, DPPH, & FRAP Serta Kolerasinya dengan Fenol dan Flavonoid pada Enam Tanaman”, Skripsi, FMIPA Institut Pertanian, Bogor, 2010.
[6] Pamungkas, J. D., Anam, K., & Kusrini, D., “Penentuan Total Kadar Fenol dari Daun Kersen Segar, Kering dan Rontok (Muntingia calabura L.) serta Uji Aktivitas Antioksidan dengan Metode DPPH”. Jurnal Kimia Sains Dan Aplikasi, 2016; vol. 19, no. 1, pp. 15–20, 2016.
[7] Megawati, Aswad, M., Yohanes D.P., Embu, A., Khadijah. “Uji Aktivitas Antioksidan Ekstrak Etanol Daun Murbei (Morrus alba L) Asal Kupang, Nusa Tenggara Timur dengan Metode DPPH”. TECHNO: Jurnal Penelitian, 2019; vol. 08, no. 1.
[8] Nurmiati, “Uji Aktivitas Antioksidan Ekstrak Etanol dan Ekatrak Air dari Seledri (Aplum Graviolens L.)”. Skripsi. Palu Universitas Tadulako, 2018.
[9] Widyani, M., Ulfa, M., & Wirasisya, D. G. “Efek Penghambatan Radikal Bebas Infusa dan Ekstrak Etanol Herba Pegagan (Centella Asiatica (L.) Urb) Dengan Metode DPPH”, Jurnal Pijar Mipa, 2019; vol. 14, no. 1, pp. 100- 106.
[10] Ergina, Nuryanti, S. dan Pursitasari, D., “Uji Kualitatif Senyawa Metabolit Sekunder Pada Daun Palado (Agave Angustifolia) Yang Diekstraksi Dengan Pelarut Air Dan Etanol Qualitative Test of Secondary Metabolites Compounds in Palado Leaves ( Agave Angustifolia ) Extracted With Water and Ethanol”, Akademika Kimia, 204; vol. 3, no. 8, pp. 165–172.
[11] Rizkayanti, Diah, A. W. M., & Jura, M. R., “Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Etanol Daun Kelor (Moringa Oleifera LAM)”. Jurnal Akademika Kimia, 2017; vol. 6, no. 2, pp. 125–131.
[12] Yati, J. S., Sumpono, & Candra, N. I., Potensi Aktivitas Antioksidan Metabolit Sekunder dari Bakteri Endofit pada Daun Moringa oleifera L. Jurnal Pendidikan Dan Ilmu Kimia, 2018; vol. 2, No. 1, pp. 82–87.
[13] Sulaiman ISC, Basri M, Masoumi HRF, Chee WJ, Ashari SE, Ismail M., “Effects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology”. Chem Cent J, 2017, vol. 11, pp. 54, 2017.
[14] Yuliani, N.N. dan Dienina, D.P., “Uji Aktivitas Antioksidan Infusa Daun Kelor (Moringa oleifera Lamk) Dengan Metode DPPH”. Jurnal Info Kesehatan, 2015; vol. 14, No. 2, pp. 1060-1082.
[15] Kuncoro, H., Mutia N. S., Hifdzur, R. R., Risna A., “Evaluasi Parameter Mutu Ekstrak Air Daun Tahongai (Kleinhovia hospita Linn)”, Pharmaceutical Journal of Indonesia, 2022; vol. 7, no. 2, pp. 115 – 122.
[16] Edy, U., Supriadi, Anang, W. D, Jura, M. R., “Uji Aktivitas Antioksidan Ekstrak Etanol Daun Balaroa (Kleinhovia hospita L.) Menggunakan DPPH”, Media Eksakta, 2023; vol. 19, no. 1, pp. 83-89.
[17] Hasanuddin, S., & Andini, C., “Uji Aktivitas Antiradikal Bebas Ekstrak Daun Paliasa (Kleinhovia hospita Linn.)”. Jurnal Mandala Pharmacon Indonesia, 2017; vol. 3, no. 02, pp. 119–126.
[18] Saputra, S. H., Kurniawati, Nurlina, S., “Bubuk Instan daru Ekstrak Tahongai (Kleinhovia hospital)”. Prosiding Seminar Nasional Balai Riset dan Standardisasi Industri Samarinda, 2017, vol. 1, pp. 352-358.
[19] Hardiningtyas, S. D., Purwaningsih, S., Handharyani, E., “Antioxidant Activity and Hepatoprotective Effect of Green Mangrove Leaves”, JPHPI, 2017, vol. 17, no. 1, pp. 80-91.
[20] Hasan, H., Nur A. T., Faramita H., Fika N. R. Putri A. S. I. “Skrining Fitokimia dan Uji Aktivitas Antioksidan Kulit Batang Matoa (Pometia pinnata) dengan Metode 1,1-Diphenyl-2 picrylhidrazyl (DPPH)”. Indonesian Journal of Pharmaceutical Education, 2022; vol. 2, no. 1, pp. 52-56.
Published
2024-07-21
How to Cite
LeswanaN., & SianturiS. (2024). Antioxidant Activity of Tahongai Leaves (Klenhovia hospital L.) Infusa Using DPPH Method. JPK : Jurnal Proteksi Kesehatan, 13(1), 36-45. https://doi.org/10.36929/jpk.v13i1.791