Antioxidant Activity Assay of Black Turmeric (C. Caesia) Water and Ethanol Extracted Using DPPH
Keywords:
C.caesia, Antioxidant, Water, Methanol
Abstract
Antioxidants are compounds that provide electrons; they function by donating an electron to oxidizing compounds, thereby inhibiting the activity of these oxidants. The objective of this study is to ascertain the presence of secondary metabolites and evaluate the antioxidant properties of water and methanol extracts obtained from black turmeric (C. caesia). The determination of antioxidant activity was conducted using the DPPH method with a microplate reader at a wavelength of 520 nm, by calculating the IC50 value. The research findings indicate that both water and methanol extracts contain flavonoids, saponins, and tannins. The antioxidant activity of the water extract measured at 392.47 ± 46.38 ppm (weaker), the methanol extract displayed a higher activity at 69.66 ± 2.91 ppm (strong), and Vitamin C exhibited very strong activity at 3.98 ± 0.26 ppm.
References
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[3] Lesjak M, Beara I, Simin N, et al. Antioxidant and anti-inflammatory activities of quercetin and its derivatives. J Funct Foods, 2018
[4] Rahaman ST, Mondal S. “Flavonoids: A vital resource in healthcare and medicine”. Pharm Pharmacol Int J, vol. 8, no. 2, pp. 91-104, 2020. doi:10.15406/ppij.2020.08.00285
[5] Mangla, M., Shuaib, M., Jain, J., & Kashyap, M. “In-vitro Evaluation of antioxidant activity of Curcuma caesia Roxb”. Internation Journal of Pharmaceutical Sciences and Research, vol. 1, no. 9, pp. 98–102, 2010.
[6] Rachmawati.A, Ni Wayan Wisaniyasa, I Ketut Suter. “Pengaruh Jenis Pelarut Terhadap Aktivitas Antioksidan Ekstrak Meniran (Phyllanthus niruri L.). Jurnal Itepa, vol. 9, no. 4, pp. 458-467, 2020.
[7] Wardaniati, I., & Yanti, R. “Uji Aktivitas Antioksidan Ekstrak Etanol Propolis Lebah Trigona (Trigona itama) menggunakan Metode DPPH”. JOPS (Journal Of Pharmacy and Science), vol. 2, no. 1, pp. 14–21, 2020. https://doi.org/10.36341/jops.v2i1.1257
[8] Sulastri, L., Oktavia, I., & Simanjuntak, P. Aktivitas Antioksidan Kecibeling, Bakau Merah, Dan Katuk Pada Metode Ekstraksi Dan Rasio Ekstrak Yang Berbeda. Buletin Penelitian Tanaman Rempah Dan Obat, vol. 31, no. 1, 2020. https://doi.org/10.21082/bullittro.v31n1.2020.1-7
[9] Islami, D., Pratiwi, D., Mardhiyani, D., & Farmasi dan Ilmu Kesehatan, F. “Phytochemical Screening of Turmeric (Curcuma domestica Val) and Red Ginger (Zingiber officinale Var Roscoe) Rhizomes Infusion Skrining Fitokimia Infusa Rimpang Kunyit (Curcuma domestica Val) dan Rimpang Jahe Merah (Zingiber officinale var roscoe)”. Jurnal Proteksi Kesehatan, vol. 11, no. 1, pp. 1–6, 2022.
[10] Zhang, Q., Zhang, J., Shen, J., Silva, A., Dennis, D. A., & Barrow, C. J. “A simple 96-well microplate method for estimation of total polyphenol content in seaweeds”. Journal of Applied Phycology, vol. 18, no. 3-5, pp. 445–450, 2006 https://doi.org/10.1007/s10811-006-9048-4
[11] Nurjannah, I., Mustariani, B. A. A., & Suryani, N. Skrining Fitokimia dan “Uji Antibakteri Ekstrak Kombinasi Daun Jeruk Purut (Citrus hystrix) dan Kelor (Moringa oleifera l.) Sebagai Zat Aktif pada Sabun Antibakteri”. Spin, vol. 4, no. 1, pp. 23–36, 2022.
[12] Ling.T,Lucinda Boyd and Fatima Rivas. “Triterpenoids as Reactive Oxygen Species Modulators of Cell Fate”, Cem Res Toxico, 35(4): 569–584 Pub Med, 2022.
[13] Sangi, M., Runtuwene, M. R. J., & Simbala, H. E. I. “Analisa Fitokimia Obat Di Minahasa Utara”. Chemistry Progres, vol. 1, no. 1, pp. 47–53, 2008.
[14] Marliana, S. D., Suryanti, V., & Suyono. “Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Kimia Buah Labu Siam (Sechium edule Jacq. Swartz.) dalam Ekstrak Etanol”. Biofarmasi, vol. 3, no. 1, pp. 26–31, 2005.
[15] Ni Nyoman Yulaini, J. Sambara, M. Alexandria Mau. Uji Aktivitas Antioksidan Fraksi Etilasetat Ekstrak Rimpang Jahe Merah (Zingiber officinale var. Rubrum) Dengan Metode DPPH (1,1-Diphenyl-2-picrylhydrazyl). Jurnal info Kesehatan, vol. 14, no. 1, pp. 1091-1111, 2016.
[16] Zuhra, C. F., Tarigan, J. B., & Sihotang, H. “Aktivitas Antioksidan Senyawa Flavonoid DAri Daun Katuk (Sauropus androgunus (L) Merr.)”. Jurnal Biologi Sumatra, vol. 3, no. 1, pp. 10–13, 2008.
[17] Theafelicia Z, Wulan SN. “Comparison of Various Methods for Testing Antioxidant Activity (DPPH, ABTS, and FRAP) on Black Tea (Camellia sinensis) Zerlinda”. J Teknol Pertan, vol. 24, no. 1, pp. 35-44, 2023.
[18] Surya, A., & Rahayu, D. P. “Antioksidan Ekstrak Metanol Kulit Petai (Parkia speciosa Hassk) dengan Metode 2,2-diphenyl-1-picrylhidrazyl. JOPS (Journal Of Pharmacy and Science), vol. 4, no. 2, pp. 1–5, 2020. https://doi.org/10.36341/jops.v4i2.1342
[19] Sari, A. N. “Potensi Antioksidan Alami Pada Ekstrak Daun Jamblang (Syzigium cumini (L.) Skeels)”. EKSAKTA: Berkala Ilmiah Bidang MIPA, vol. 18, no. 2, pp. 107–112, 2017. https://doi.org/10.24036/eksakta/vol18-iss02/61
[20] Hasan, H., Ain Thomas, N., Hiola, F., & Ibrahim, A. S. “Skrining Fitokimia dan Uji Aktivitas Antioksidan Kulit Batang Matoa (Pometia pinnata) Dengan Metode 1,1-Diphenyl-2 picrylhidrazyl (DPPH)”. Indonesian Journal of Pharmaceutical Education, vol. 1, no. 3, pp. 67–73, 2022. https://doi.org/10.37311/ijpe.v2i1.10995
[21] Andriani, D., & Murtisiwi, L. Uji Aktivitas Antioksidan Ekstrak Etanol 70% Bunga Telang (Clitoria ternatea L) dari Daerah Sleman dengan Metode DPPH Antioxidant Activity Test of 70% Ethanol Extract of Telang Flower (Clitoria ternatea L) from Sleman Area with DPPH Method.” Jurnal Farmasi Indonesia, vol. 17, no. 1, pp. 70–76, 2020.
[22] FT, Mhaisen, & Et, A. “Uji Aktivitas Antioksidan Ekstrak Etanol Daun Karika (Carica Pubescens) Dengan Metode Dpph Beserta Identifikasi Senyawa Alkaloid, Fenol Dan Flavonoid”. Angewandte Chemie International Edition, vol. 6, no. 11, pp. 951–952, 2018
Published
2023-12-08
How to Cite
Kartini, S., Susanti, P., Marshanda, I., Pratiwi, D., Mardhiyani, D., & Hendrika, Y. (2023). Antioxidant Activity Assay of Black Turmeric (C. Caesia) Water and Ethanol Extracted Using DPPH. JPK : Jurnal Proteksi Kesehatan, 12(2), 163-168. https://doi.org/10.36929/jpk.v12i2.790
Section
Articles
